Skip to main content

Validation of a PCR method for detection on poultry packs

Buy Article:

$46.50 plus tax (Refund Policy)


This study describes a multiplex polymerase chain reaction (PCR) detection system combined with enrichment growth conditions for simultaneous detection and identification of C. jejuni, C. coli and thermotolerant Campylobacter in poultry pack rinses. The PCR primers were tested against a range of Campylobacter and non-Campylobacter species, and PCR products were only amplified from target organisms. The sensitivity of the method was similar to that obtained by conventional plating procedures, but when used in combination with the MPN method of enumeration could detect levels down to 6?MPN/100?ml of rinse. The validation of 50 samples of chicken pack rinses demonstrated the versatility of this approach in microbiological surveys to yield data for risk assessments.

Keywords: Food products; Food safety; Food testing; Micro-organisms

Document Type: Research Article


Publication date: 2004-09-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more