Antioxidant supplementation lowers circulating IGF-1 but not F₂-isoprostanes immediately following anterior cruciate ligament surgery

Authors: Barker, Tyler¹; Leonard, Scott W.²; Trawick, Roy H.³; Walker, James A.⁴; Traber, Maret G.⁵

Source: Redox Report, Volume 14, Number 5, October 2009 , pp. 221-226(6)

Publisher: Maney Publishing

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Abstract:

Interleukin (IL)-10 is an anti-inflammatory cytokine that suppresses pro-inflammatory cytokines. We previously demonstrated that supplementation with vitamins E and C ameliorated the increase in IL-10 immediately following anterior cruciate ligament (ACL) surgery in the absence of other cytokine perturbations. Since both oxidative stress and insulin-like growth factor-1 (IGF-1) can modulate IL-10 concentrations, the mechanisms for these changes warranted further investigation. Our objective was to evaluate the mechanism for the IL-10 decrease following ACL surgery. This study consisted of randomized, double-blind, placebo-controlled experimental design. Subjects were randomly assigned to daily supplementation with either: (i) antioxidants (AO; vitamins E [α-tocopherol] and C [ascorbic acid]; n = 10); or (ii) matching placebos (PL; n = 10). Supplementation started ∼2 weeks prior to surgery (baseline) and concluded 3 months after surgery. Subjects provided six fasting blood samples at: (i) baseline; (ii) immediately pre-surgery (Pre); (iii) 90 min; (iv) 72 h; (v) 7 days; and (vi) 3 months post-surgery. α-Tocopherol, ascorbic acid, F₂-isoprostane and IGF-1 concentrations were measured in each blood sample. At 90 min relative to other times, plasma F₂-isoprostane concentrations were significantly (P < 0.05) elevated in both groups, while at 90 min IGF-1 was significantly (P < 0.05) lower in the AO compared to the PL group. The changes in IGF-1 at 90 min relative to baseline were correlated (P < 0.0001) with the changes in IL-10. The decrease in IL-10 observed in the AO group is likely dependent on the decrease IGF-1 since lipid peroxidation was unchanged between the two groups.

Keywords: ANTERIOR CRUCIATE LIGAMENT; VITAMIN E; VITAMIN C; OXIDATIVE STRESS; INSULIN-LIKE GROWTH FACTOR1; CYTOKINES

Document Type: Research article

DOI: 10.1179/135100009X12525712409535

Affiliations: 1: Sport Science Department, The Orthopedic Specialty Hospital, Murray, Utah, USA; Department of Nutrition and Exercise Sciences, Oregon State University, Corvallis, Oregon, USA; Linus Pauling Institute, Oregon State University, Corvallis, Oregon, U 2: Linus Pauling Institute, Oregon State University, Corvallis, Oregon, USA 3: Sports Medicine Department, The Orthopedic Specialty Hospital, Murray, Utah, USA; The Orthopedic Specialty Clinic, Murray, Utah, USA 4: Sport Science Department, The Orthopedic Specialty Hospital, Murray, Utah, USA 5: Department of Nutrition and Exercise Sciences, Oregon State University, Corvallis, Oregon, USA; Linus Pauling Institute, Oregon State University, Corvallis, Oregon, USA

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