Antioxidant supplementation lowers circulating IGF-1 but not F2-isoprostanes immediately following anterior cruciate ligament surgery

Authors: Barker, Tyler1; Leonard, Scott W.2; Trawick, Roy H.3; Walker, James A.4; Traber, Maret G.5

Source: Redox Report, Volume 14, Number 5, October 2009 , pp. 221-226(6)

Publisher: Maney Publishing

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content

Abstract:

Interleukin (IL)-10 is an anti-inflammatory cytokine that suppresses pro-inflammatory cytokines. We previously demonstrated that supplementation with vitamins E and C ameliorated the increase in IL-10 immediately following anterior cruciate ligament (ACL) surgery in the absence of other cytokine perturbations. Since both oxidative stress and insulin-like growth factor-1 (IGF-1) can modulate IL-10 concentrations, the mechanisms for these changes warranted further investigation. Our objective was to evaluate the mechanism for the IL-10 decrease following ACL surgery. This study consisted of randomized, double-blind, placebo-controlled experimental design. Subjects were randomly assigned to daily supplementation with either: (i) antioxidants (AO; vitamins E [α-tocopherol] and C [ascorbic acid]; n = 10); or (ii) matching placebos (PL; n = 10). Supplementation started ∼2 weeks prior to surgery (baseline) and concluded 3 months after surgery. Subjects provided six fasting blood samples at: (i) baseline; (ii) immediately pre-surgery (Pre); (iii) 90 min; (iv) 72 h; (v) 7 days; and (vi) 3 months post-surgery. α-Tocopherol, ascorbic acid, F2-isoprostane and IGF-1 concentrations were measured in each blood sample. At 90 min relative to other times, plasma F2-isoprostane concentrations were significantly (P < 0.05) elevated in both groups, while at 90 min IGF-1 was significantly (P < 0.05) lower in the AO compared to the PL group. The changes in IGF-1 at 90 min relative to baseline were correlated (P < 0.0001) with the changes in IL-10. The decrease in IL-10 observed in the AO group is likely dependent on the decrease IGF-1 since lipid peroxidation was unchanged between the two groups.

Keywords: ANTERIOR CRUCIATE LIGAMENT; VITAMIN E; VITAMIN C; OXIDATIVE STRESS; INSULIN-LIKE GROWTH FACTOR1; CYTOKINES

Document Type: Research article

DOI: 10.1179/135100009X12525712409535

Affiliations: 1: Sport Science Department, The Orthopedic Specialty Hospital, Murray, Utah, USA; Department of Nutrition and Exercise Sciences, Oregon State University, Corvallis, Oregon, USA; Linus Pauling Institute, Oregon State University, Corvallis, Oregon, U 2: Linus Pauling Institute, Oregon State University, Corvallis, Oregon, USA 3: Sports Medicine Department, The Orthopedic Specialty Hospital, Murray, Utah, USA; The Orthopedic Specialty Clinic, Murray, Utah, USA 4: Sport Science Department, The Orthopedic Specialty Hospital, Murray, Utah, USA 5: Department of Nutrition and Exercise Sciences, Oregon State University, Corvallis, Oregon, USA; Linus Pauling Institute, Oregon State University, Corvallis, Oregon, USA

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$51.00 plus tax      Refund Policy

 

OR

Back to top

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages.
Page Help Click here for Page Help
Shopping cart
Tools
Sign in






Need to register?
Sign up here
Text size: A | A | A | A