Buffer modulation of menadione-induced oxidative stress in Saccharomyces cerevisiae
Authors: Lushchak, Oleh V.1; Bayliak, Maria M.1; Korobova, Olha V.1; Levine, Rodney L.2; Lushchak, Volodymyr I.1
Source: Redox Report, Volume 14, Number 5, October 2009 , pp. 214-220(7)
Publisher: Maney Publishing
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Abstract:
The objective of this study was to compare, in vivo, the effects of bicarbonate and phosphate buffers on survival and menadione-induced oxidative stress in yeast cells. The latter were treated with different concentrations of menadione in the presence of these two buffers. At 25 mM concentration of buffers, menadione only slightly reduced yeast surviving; at 50 mM concentration, cell killing by menadione was much more pronounced in bicarbonate than in phosphate buffer. Although the content of protein carbonyl groups did not show development of oxidative stress under menadione-induced stress, inactivation of aconitase and decrease in glutathione level mirrored its induction. However, cellular glutathione and aconitase activity decrease did not correlate with yeast survival. In vitro, aconitase was more quickly inactivated in 50 mM carbonate, than in 50 mM phosphate buffer. The possible involvement of the carbonate radical in these processes is discussed.Keywords: ACONITASE; ANTIOXIDANT ENZYMES; GLUTATHIONE; PROTEIN CARBONYLS; REACTIVE OXYGEN SPECIES; SACCHAROMYCES CEREVISIAE
Document Type: Research article
DOI: 10.1179/135100009X12525712409454
Affiliations: 1: Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine 2: Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
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