Improved peroxyl radical scavenging TOSC assay to quantify antioxidant capacity using SIFT-MS
Authors: Senthilmohan, Senti T.1; Davis, Brett M.1; Wilson, Paul F.2; McEwan, Murray J.1
Source: Redox Report, Volume 14, Number 5, October 2009 , pp. 197-204(8)
Publisher: Maney Publishing
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Abstract:
We report a new, fast, sensitive variation of the total oxyradical scavenging capacity (TOSC) assay for measuring the antioxidant capacity of pure compounds, plant extracts and biological fluids using selected ion flow tube mass spectrometry (SIFT-MS). The TOSC assay examines the partial inhibition of ethene formation in the presence of antioxidants that compete with α-keto-γ-methiolbutyric acid (KMBA) for reactive oxygen species. The SIFT-MS-TOSC assay takes 15 s for each ethene analysis and the time interval between consecutive analyses is 20 s. We demonstrate the method by monitoring the antioxidant capacity of several standard radical scavengers of peroxyl radicals. For peroxyl radicals the measured SIFT-MS-TOSC concentrations necessary to produce 50% inhibition of radical reaction with KMBA are 6.1 ± 0.3 μM for Trolox, 5.7 ± 0.3 μM for ascorbic acid, 8.4 ± 0.4 μM for uric acid and 38 ± 2 μM for reduced glutathione.Keywords: TOSC ASSAY; ANTIOXIDANT CAPACITY; SIFT-MS
Document Type: Research article
DOI: 10.1179/135100009X12525712409571
Affiliations: 1: Department of Chemistry, University of Canterbury, Christchurch, New Zealand; Syft Technologies Ltd, Christchurch, New Zealand 2: Department of Chemistry, University of Canterbury, Christchurch, New Zealand
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