Home >> Redox Report, Volume 12, Number 4

Time course and attenuation of ischaemia-reperfusion induced oxidative injury by propofol in human renal transplantation

Authors: Basu, Samar1; Meisert, Isabelle2; Eggensperger, Elisabeth2; Krieger, Elisabeth2; Krenn, Claus2

Source: Redox Report, Volume 12, Number 4, August 2007 , pp. 195-202(8)

Publisher: Maney Publishing

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Abstract:

Ischaemia-reperfusion injury resulting from interruption and restoration of blood flow might be related to free radical mediated oxidative stress and inflammation, and subsequently to post-surgery related complications. We studied the impact of renal transplantation on oxidative stress and inflammation by measuring F2-isoprostanes and prostaglandin F2?, respectively, during transplantation and post-surgery. Additionally, due to earlier observations, two dissimilar anaesthetic agents (thiopentone and propofol) were compared to determine their antioxidative capacity rather than their anaesthetic properties. Blood samples were collected before, post-intubation, immediately, 30, 60,120, 240 min, and 12 and 24 h after reperfusion. Oxidative stress and inflammatory response were detected by measuring 8-iso-PGF2? (a major F2-isoprostane and a biomarker of oxidative stress) and 15-keto-dihydro-PGF2? (a major metabolite of PGF2? and a biomarker of COX-mediated inflammatory response), respectively. Reperfusion of the transplanted graft significantly increased plasma levels of 8-iso-PGF2?. PGF2? metabolite levels, although elevated, did not reach statistical significance. In addition, significantly lower levels of 8-iso-PGF2a were observed in the propofol group compared to the thiopentone group. Together, these findings underline an augmented oxidative stress activity following an inflammatory response after human renal transplantation. Furthermore, propofol a well-known anaesthetic, counteracted oxidative stress by lowering the formation of a major F2-isoprostane.

Keywords: OXIDATIVE STRESS; INFLAMMATION; ISOPROSTANES; ISCHAEMIA-REPERFUSION; PROSTAGLANDINS; RENAL TRANSPLANTATION; PROPOFOL

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/135100007X200281

Affiliations: 1: Clinical Nutrition and Metabolism, Department of Public Health and Caring Sciences, Faculty of Medicine, Uppsala University, Uppsala, Sweden 2: Department of Anaesthesiology & Intensive Care Medicine, University of Vienna, Vienna, Austria

Publication date: 2007-08-01

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