Yeast flavohemoglobin protects against nitrosative stress and controls ferric reductase activity

Authors: Lewinska, Anna¹; Bartosz, Grzegorz²

Source: Redox Report, Volume 11, Number 5, October 2006 , pp. 231-239(9)

Buy & download fulltext article:

Price: $48.00 plus tax (Refund Policy)

Abstract:

The role of Saccharomyces cerevisiae flavohemoglobin (Yhb1) is controversial and far from understood. This study compares the effects of nitrosative and oxidative challenge on the yeast mutant lacking the YHB1 gene. Growth of the mutant was impaired by nitrosoglutathione and peroxynitrite, whereas increased sensitivity to reactive oxygen species was not observed. Increased levels of intracellular NO^• after incubation with NO^• donors were found in the mutants cells as compared to the wild-type cells. Deletion of the YHB1 gene was found to augment the reduction of Fe³⁺ by yeast cells which suggests that flavohemoglobin participates in regulation of the activity of plasma membrane ferric reductase(s).

Keywords: FERRIC REDUCTASE; SACCHAROMYCES CEREVISIAE; YEAST; FLAVOHEMOGLOBIN; NITROSATIVE STRESS; NITRIC OXIDE; PEROXYNITRITE

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/135100006X154987

Affiliations: 1: Department of Biochemistry and Cell Biology, University of Rzeszow, Rzeszow, Poland 2: Department of Biochemistry and Cell Biology, University of Rzeszow, Rzeszow, Poland; Department of Molecular Biophysics, University of Lodz, Lodz, Poland

Publication date: 2006-10-01

More about this publication?