@article {Jarrett:August 2006:1351-0002:144,
	author = "Jarrett, Stuart G.",
	author = "Cuenco, Joy",
	author = "Boulton, Mike",
	title = "Dietary antioxidants provide differential subcellular protection in epithelial cells",
	journal = "Redox Report",
	volume = "11",
	year = "August 2006",
	abstract = "This study aimed to evaluate the organelle-specific antioxidant/pro-oxidant actions of clinically important dietary antioxidants against oxidative stress. An <I>in vitro</I> cellular model was employed to investigate the antioxidant/pro-oxidant effects of various concentrations (1, 10 and 100 &#956;M) of ascorbic acid, &#945;-tocopherol and &#946;-carotene during H<SUB>2</SUB>O<SUB>2</SUB>-induced oxidative stress. Damage to nuclear and mitochondrial genomes was analyzed by quantitative polymerase chain reaction and oxidation of membrane lipids was measured via colorimetric assays. The key findings were: (i) dietary antioxidants conferred a dose-dependent protective effect (with a pro-oxidant shift at higher concentrations); (ii) the protection conferred to different sub-cellular organelles is highly specific to the dietary antioxidant; (iii) the mtDNA is highly sensitive to oxidative attack compared to nDNA (<I>P</I> &#60; 0.05); and (iv) mtDNA protection conferred by dietary antioxidants was required to improve protection against oxidative-induced cell death. This study shows that antioxidant-induced protection of mtDNA is an important target for future oxidative stress therapies.",
	pages = "144-152(9)",
	url = "http://www.ingentaconnect.com/content/maney/rer/2006/00000011/00000004/art00001"
	doi = "doi:10.1179/135100006X116646"
}