Home >> Redox Report, Volume 9, Number 6

Rotenone, a mitochondrial electron transport inhibitor, ameliorates ischemia–reperfusion-induced intestinal mucosal damage in rats

Authors: Ichikawa, Hiroshi1; Takagi, Tomohisa2; Uchiyama, Kazuhiko2; Higashihara, Hiroshi2; Katada, Kazuhiro2; Isozaki, Yutaka2; Naito, Yuji3; Yoshida, Norimasa3; Yoshikawa, Toshikazu2

Source: Redox Report, Volume 9, Number 6, December 2004 , pp. 313-316(4)

Publisher: Maney Publishing

Buy & download fulltext article:

OR

Price: $48.00 plus tax (Refund Policy)

Abstract:

In ischemia–reperfusion (I/R)-induced tissue injury, oxygen radicals can be generated by several mechanisms. One of the important sources of oxygen radicals is thought to be mitochondrial respiration. The aim of this study was to investigate the antioxidative defense effect of the mitochondrial electron transport inhibitor, rotenone using the I/R-induced rat intestinal mucosal injury model in vivo. Intestinal ischemia was induced for 30 min by applying a small clamp to the superior mesenteric artery in rats. Rotenone at a dose of 100 mg/kg was given to rats orally 2 h before the ischemia. Intraluminal hemoglobin and protein levels, the mucosal content of thiobarbituric acid-reactive substances (TBARS), the mucosal myeloperoxidase activity, and the content of inflammatory cytokines (CINC-1, TNF-?) were all significantly increased from mean basal levels after 60 min of reperfusion. These increases after I/R were inhibited by treatment with rotenone at a dose of 100 mg/kg. Co-administration with succinate (100 mg/kg), a substrate of the mitochondrial electron transport system, cancelled significant reduction of intraluminal hemoglobin and mucosal TBARS treated with rotenone alone. The results of the present study indicate that rotenone inhibited lipid peroxidation and reduced development of the intestinal mucosal inflammation induced by I/R in rats. This investigation suggests that rotenone has potential as a new therapeutic agent for reperfusion injury.

Keywords: RAT; ISCHEMIA-REPERFUSION; INTESTINAL MUCOSAL DAMAGE; ROTENONE; REPERFUSION INJURY

Document Type: Regular Paper

DOI: http://dx.doi.org/10.1179/135100004225006795

Affiliations: 1: Department of Food Sciences and Nutritional Health, The Faculty of Human Environment, Kyoto Prefectural University, Kyoto, Japan 2: Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan 3: Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan

Publication date: 2004-12-01

Marked list

Tools

Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content

Text size:

A | A | A | A
^ Back to top
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages. print icon Print this page