Assessment of membrane protection by traditional Chinese medicines using a flow cytometric technique: preliminary findings
Authors: Chung, Wai Y.; Yow, Christine M.N.; Benzie, Iris F.F.
Source: Redox Report, Volume 8, Number 1, February 2003 , pp. 31-33(3)
Publisher: Maney Publishing
Abstract:In this preliminary study, we used a 'living cell' flow-cytometric approach to membrane protection by four traditional Chinese medicines (TCMs). Cells were incubated, separately, for 30 min with aqueous extracts (1.5% w/v) of lingzhi (Ganoderma lucidum), ginger (Zingiber officianale), ginseng (Panax ginseng), and green tea (Camellia sinensis). Membranes were labelled with a fluorescent probe, cells were then incubated with cumene hydroperoxide, and site-specific oxidation induced by iron/ascorbate. Oxidation of membrane lipids quenches fluorescence. Forward-scatter fluorescence was measured at timed intervals after initiation of oxidation. Results indicate that lingzhi and ginger contain antioxidant component(s) that act within the cell membrane and slow lipid peroxidation in situ. Results demonstrate also that this living cell model is a useful biomonitoring tool to help determine molecular aspects of putative health effects of TCMs.
Document Type: Regular Paper
Affiliations: Department of Nursing and Health Sciences, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, China
Publication date: 2003-02-01