Protective properties of butanolic extract of the Calendula officinalis L.(marigold) against lipid peroxidation of rat liver microsomes and action as free radical scavenger
Authors: Cordova, Clarissa A.S.1; Siqueira, Ionara R.2; Netto, Carlos A.2; Yunes, Rosendo A.3; Volpato, Ana M.3; Filho, Valdir Cechinel4; Curi-Pedrosa, Rozangela5; Creczynski-Pasa, Tânia B.6
Source: Redox Report, Volume 7, Number 2, April 2002 , pp. 95-102(8)
Publisher: Maney Publishing
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Abstract:
Calendula officinalis (marigold) has many pharmacological properties. It is used for the treatment of skin disorders, pain and also as a bactericide, antiseptic and anti-inflammatory. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are known to participate in the pathogenesis of various human diseases and may be involved in the conditions which C. officinalis is used to treat. The aim of this study was to investigate the relationship between the beneficial properties of this plant and its antioxidant action. The butanolic fraction (BF) was studied because it is non-cytotoxic and is rich in a variety of bioactive metabolites including flavonoids and terpenoids. Superoxide radicals (O2•-) and hydroxyl radicals (HO•) are observed in decreasing concentrations in the presence of increasing concentrations of BF with IC50 values of 1.0 ± 0.09 mg/ml and 0.5 ± 0.02 mg/ml, respectively, suggesting a possible free radical scavenging effect. Lipid peroxidation in liver microsomes induced by Fe2+/ascorbate was 100% inhibited by 0.5 mg/ml of BF (IC50 = 0.15 mg/ml). Its total reactive antioxidant potential (TRAP) (in μM Trolox equivalents) was 368.14 ± 23.03 and its total antioxidant reactivity (TAR) was calculated to be 249.19 ± 14.5 μM. The results obtained suggest that the butanolic fraction of C. officinalis possesses a significant free radical scavenging and antioxidant activity and that the proposed therapeutic efficacy of this plant could be due, in part, to these properties.Document Type: Research article
DOI: 10.1179/135100002125000325
Affiliations: 1: Departamentos de Ciências Fisiológicas - Biofísica, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil 2: Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil 3: Departamentos de Química, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil 4: NIQFAR- Universidade do Vale do Itajaí, Itajaí, Santa Catarina, Brazil 5: Departamentos de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil 6: Departamentos de Ciências Fisiológicas - Biofísica, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil
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