Authors: Hosseini, M.¹; Ostad, N.²; Parivar, K.³; Ghahremani, M. H.⁴

Source: Neurological Research, Volume 32, Number 2, March 2010 , pp. 215-220(6)

Publisher: Maney Publishing

Abstract:

DNA damage, as an important initiator of neuronal cell death, has been implicated in numerous neurodegenerative conditions. We previously delineated several pathways that control embryonic cortical neuronal cell death evoked by the DNA-damaging agent, camptothecin. The topisomerase-1 inhibitor, camptothecin, has been shown to induce cortical neuronal cell death in a reproducible and synchronistic manner. Primary embryonic neuronal cell culture cortical neurons were prepared. In the study, the survival % of neurons in camptothecin P38 group, after 6 hours (85%), 24 hours (64%) and 48 hours (50%), compared to camptothecin ATF-2 and P38 group after 4 hours (97 and 95%), have been significantly lower, and the expression % of neurons in camptothecin P38 group , after 6 hours (20%), 24 hours (40%) and 48 hours (55%), compared to camptothecin ATF-2 and P38 group after 4 hours (5 and 3%) have been significant lower (p<0?05). The expression % of neurons in camptothecin P38 group, after 24 hours (40%), compared to camptothecin ATF-2 group after 24hours (30%), have been significant lower (p<0?05). This study revealed that camptothecin induces P38 expression and P38 in embryonic cortical neurons to determine the importance of the P38 pathway in neuronal death following DNA damage, and P38 is induce phosphorylation of ATF-2 in embryonic cortical neurons following DNA damage.

Keywords: P38; CAMPTOTHECIN; DNA DAMAGE; ATF-2; RAT EMBRYO CORTEX

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/174313209X382304

Affiliations: 1: Department of Science, Islamshahr Branch, Islamic Azad University, Tehran, Iran 2: Pharmacology Department, Tehran University, Tehran, Iran 3: Department of Animal Biology, Science and Research Campus, Islamic Azad University, Tehran, Iran 4: Pharmacology Research Lab, Room J-309, Department of Pharmacology–Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran;, Email: mhghahremani@sina.tums.ac.ir

Publication date: 2010-03-01

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