Suppression of the multidrug transporter P-glycoprotein using RNA interference in cultured rat astrocytes induced by coriaria lactone

Authors: Yang, Tian-Hua¹; Tian, Lin-Yu¹; Shang, Hui-Fang¹; Cheng, Xin-Wang¹; Geng, Jia¹; Chen, Lei¹; Zhou, Dong¹

Source: Neurological Research, Volume 31, Number 10, December 2009 , pp. 1084-1091(8)

Publisher: Maney Publishing

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Abstract:

The overexpression of the multidrug resistance gene (MDR-1) and its translational product p-glycoprotein (P-gp) may play an important role in pharmacoresistant epilepsy. We established the rat astrocyte model overexpressing P-gp induced by coriaria lactone and successfully nucleofected it with the siRNA-hairpin expression vector pSIREN-shuttle designed to target MDR-1B mRNA. The mRNA expression of MDR-1B gene was mostly knock down by 67.70% (p<0.01). The expression of P-gp in experimental group was significantly lower than that in negative control (p<0.05), and the rhodamine efflux ratio of experimental group (23.08%) was remarkably lower than that of negative control (78.35%, p<0.01). We first employed RNA interfering to the drug resistance reversal of refractory epilepsy and this may provide a new way for refractory epilepsy remedy.

Keywords: P-GLYCOPROTEIN; REFRACTORY EPILEPSY; ASTROCYTES; CORIARIA LACTONE; RNA INTERFERING

Document Type: Research article

DOI: 10.1179/174313208X319134

Affiliations: 1: Department of Neurology, West China Hospital, Sichuan University, Chengdu, China

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