IngentaConnect Injection of muscimol, a GABAa agonist into the anterior thalamic...

Authors: Kuramoto, Satoshi¹; Yasuhara, Takao¹; Agari, Takashi¹; Kondo, Akihiko¹; Matsui, Toshihiro¹; Miyoshi, Yasuyuki¹; Shingo, Tetsuro¹; Date, Isao¹

Source: Neurological Research, Volume 31, Number 4, May 2009 , pp. 407-413(7)

Publisher: Maney Publishing

Abstract:

The relationship between neurogenesis and epilepsy remains to be solved so far, although aberrant electric circuit recognized in epilepsy might be involved in neurogenesis. In this study, neurogenesis and the proliferation of astrocytes in the subgranular zone of the hippocampus were explored using unilateral amygdala-kindled rats with or without muscimol, a gamma-aminobutyric acid a (GABAa) agonist injection into the bilateral anterior thalamic nuclei (AN). Muscimol injection significantly ameliorated the behavioral scores of epilepsy without any significant alteration on the electroencephalography recorded at the stimulated basolateral amygdala, thus suggesting that muscimol injection might affect the secondary generalization, but not the initial discharge itself. The number of bromodeoxyuridine (BrdU), BrdU/doublecortin and BrdU/glial fibrillary acidic protein-positive cells in the subgranular zone of kindled animals increased markedly. Muscimol injection significantly suppressed neurogenesis, but not the proliferation of astrocyte, in the subgranular zone of the non-stimulated side, probably through the suppression of secondary generalization via AN. The results might indicate the underlying relationships between neurogenesis and epilepsy, that epileptic propagation in unilateral amygdala-kindled rats might go through AN into the contralateral side with subsequent neurogenesis, although further studies need to clarify the hypothesis.

Keywords: DEEP BRAIN STIMULATION; EPILEPSY; HIPPOCAMPUS; MUSCIMOL; NEUROGENESIS

Document Type: Research article

DOI: 10.1179/174313208X346125

Affiliations: 1: Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

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Injection of muscimol, a GABAa agonist into the anterior thalamic nucleus, suppresses hippocampal neurogenesis in amygdala-kindled rats