Authors: Itoh, Tatsuki¹; Satou, Takao²; Nishida, Shozo³; Tsubaki, Masahiro³; Hashimoto, Shigeo⁴; Ito, Hiroyuki¹

Source: Neurological Research, Volume 30, Number 4, May 2008 , pp. 430-434(5)

Publisher: Maney Publishing

Abstract:

Objective: Protein-free extracts from the inflamed skin of rabbits inoculated with vaccinia virus (Rosemorgen^? and Neurotropin^?) are widely employed to combat chronic pain and treat allergic conditions in human subjects in Japan. However, the pharmacologic mechanisms of Rosemorgen^? and Neurotropin^? remain unclear.

Methods: In this study, we examined the effects of Rosemorgen^? on L-glutamic acid (Glu)-induced cell death in N18-RE-105 neural cell line, which only possessed non-N-methyl-D-aspartate (NMDA)-type receptors.

Results: There were many large cytoplasmic cells and elongation of fivers in phosphate-buffered saline (PBS) additional group without Glu. In PBS and Glu simultaneous additional group, the survival ratio was decrease significantly compared with PBS alone group. Moreover, there were dead cells which did not have cytoplasm and aggregated nucleus. The Glu-induced cell death of N18-RE-105 cells was inhibited by both pre-treatment (24 hours before Glu treatment) and simultaneous treatment with Rosemorgen^?. There were many large cytoplasmic cells and elongation of fivers in Rosemorgen^? group.

Discussion: From this finding in N18-RE-105 cells, Rosemorgen^? was concluded to inhibit Glu-induced cell death via non-NMDA type receptors. One of the pharmacologic mechanisms of Rosemorgen^? has been clear. These results suggest that Rosemorgen^? depresses allodynia and chronic pain through interaction with non-NMDA type receptors.Objective: Protein-free extracts from the inflamed skin of rabbits inoculated with vaccinia virus (Rosemorgen^? and Neurotropin^?) are widely employed to combat chronic pain and treat allergic conditions in human subjects in Japan. However, the pharmacologic mechanisms of Rosemorgen^? and Neurotropin^? remain unclear.

Methods: In this study, we examined the effects of Rosemorgen^? on L-glutamic acid (Glu)-induced cell death in N18-RE-105 neural cell line, which only possessed non-N-methyl-D-aspartate (NMDA)-type receptors.

Results: There were many large cytoplasmic cells and elongation of fivers in phosphate-buffered saline (PBS) additional group without Glu. In PBS and Glu simultaneous additional group, the survival ratio was decrease significantly compared with PBS alone group. Moreover, there were dead cells which did not have cytoplasm and aggregated nucleus. The Glu-induced cell death of N18-RE-105 cells was inhibited by both pre-treatment (24 hours before Glu treatment) and simultaneous treatment with Rosemorgen^?. There were many large cytoplasmic cells and elongation of fivers in Rosemorgen^? group.

Discussion: From this finding in N18-RE-105 cells, Rosemorgen^? was concluded to inhibit Glu-induced cell death via non-NMDA type receptors. One of the pharmacologic mechanisms of Rosemorgen^? has been clear. These results suggest that Rosemorgen^? depresses allodynia and chronic pain through interaction with non-NMDA type receptors.

Keywords: CHRONIC PAIN; N18-RE-105; NON-NMDA-TYPE RECEPTOR; VACCINIA VIRUS; L-GLUTAMIC ACID

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/016164107X251763

Affiliations: 1: Department of Pathology, Kinki University School of Medicine, Osaka, Japan 2: Department of Pathology, Kinki University School of Medicine, Osaka, Japan; Division of Sports Medicine, Institute of Life Science, Kinki University, Osaka, Japan; Division of Hospital Pathology, Hospital of Kinki University School of Medicine, Osaka, Japan 3: Kinki University School of Pharmaceutical Sciences, Osaka, Japan 4: Department of Pathology, PL Hospital, Osaka, Japan

Publication date: 2008-05-01

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