Neuroprotective effect by Dammishimgyu-herbal acupuncture against H₂O₂-induced apoptosis in human neuroblastoma, SH-SY5Y cells

Authors: Park, Hi-Joon¹; Kim, YounJung¹; Kim, HakJae¹; Ha, Eunyoung¹; Park, HaeJeong¹; Yoon, SeoHyun¹; Kim, Mi-Ja²; Choi, SunMi³; Ryu, YeonHee³; Park, Hun-Kuk¹; Hong, MeeSuk¹

Source: Neurological Research, Volume 29, Supplement 1, February 2007 , pp. 93-97(5)

Publisher: Maney Publishing

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Abstract:

Background: The free radical is involved in neuronal cell death in human neurodegenerative disease. Dammishimgyu (DMSG)-herbal acupuncture has been used to treat neurological disorders in Korea. The present study was aimed to investigate the neuroprotective effect of DMSG-herbal acupuncture against H₂O₂-induced apoptosis in human neuroblastoma cell line, SH-SY5Y.

Methods: The neuroprotective effect of DMSG-herbal acupuncture on H₂O₂ induced apoptosis was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, reverse transcription-polymerase chain reaction (RT-PCR), western blots and nitrite assay.

Results: In this study, 100 ?M H₂O₂-treated cells decreased the cell viability with apoptotic features and increased the production of nitric oxide (NO). However, 0.1% DMSG treatment after exposure to 100 ?M H₂O₂ inhibited both H₂O₂-stimulated mRNA and protein expressions of BCL2-associated X protein (BAX) and caspase 3 apoptosis-related cysteine peptidase (CASP3). In addition, 0.1% DMSG treatment inhibited the increased NO production induced by H₂O₂.

Conclusion: These results suggest that DMSG-herbal acupuncture shows protective effect against H₂O₂-induced neuronal damage.

Keywords: APOPTOSIS; DAMMISHIMGYU; HERBAL ACUPUNCTURE; SH-SY5Y; NEUROPROTECTION

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/016164107X172329

Affiliations: 1: TKM Research Group, Kyung Hee University, Seoul 130-701, Korea 2: Department of Obesity Management, Graduate School of Obesity Science, Dongduk Women's University, Seoul 136-714, Korea 3: Department of Medical Research, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea

Publication date: 2007-02-01

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