Authors: Rafols, José A.; Morgan, Randy; Kallakuri, Srinivas; Kreipke, Christian W.

Source: Neurological Research, Volume 29, Number 4, June 2007 , pp. 348-355(8)

Publisher: Maney Publishing

Abstract:

Objectives: We sought to determine the extent of nerve cell injury in the Marmarou's acceleration impact model of diffuse brain injury.

Methods: Sensitive markers for cell injury including immunostaining for ?-amyloid precursor protein (?-APP, a marker for diffuse axonal injury, DAI), Fluoro-Jade (FJ) histochemistry and electron microscopy (EM) were used in sham-operated and traumatized brains.

Results: APP immunostaining confirmed and extended previous findings of DAI in association and subcortical fiber systems in the white matter after injury. Increasing FJ labeling of neurons in layers II?III of sensorimotor cortex (smCx) from 4 to 48 hours after trauma and scattered labeled cells were found in the lower cortical layers. EM confirmed the presence of dystrophic pyramidal neurons in layers II?III of smCx 24 and 48 hours post-trauma.

Discussion: Taken together, the data revealed significant nerve cell injury without apparent cell death in this model.

Keywords: CELL DEATH; DIFFUSE AXONAL INJURY; TRAUMATIC BRAIN INJURY; DIFFUSE BRAIN INJURY

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/016164107X204657

Affiliations: Department of Anatomy and Cell Biology, Scott Hall, Room No. 9312, Wayne State University School of Medicine, 540 E. Canfield, Detroit, MI 48201, USA

Publication date: 2007-06-01

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