Extent of nerve cell injury in Marmarou's model compared to other brain trauma models
Authors: Rafols, Josť A.; Morgan, Randy; Kallakuri, Srinivas; Kreipke, Christian W.
Source: Neurological Research, Volume 29, Number 4, June 2007 , pp. 348-355(8)
Publisher: Maney Publishing
Abstract:Objectives: We sought to determine the extent of nerve cell injury in the Marmarou's acceleration impact model of diffuse brain injury.
Methods: Sensitive markers for cell injury including immunostaining for ?-amyloid precursor protein (?-APP, a marker for diffuse axonal injury, DAI), Fluoro-Jade (FJ) histochemistry and electron microscopy (EM) were used in sham-operated and traumatized brains.
Results: APP immunostaining confirmed and extended previous findings of DAI in association and subcortical fiber systems in the white matter after injury. Increasing FJ labeling of neurons in layers II?III of sensorimotor cortex (smCx) from 4 to 48 hours after trauma and scattered labeled cells were found in the lower cortical layers. EM confirmed the presence of dystrophic pyramidal neurons in layers II?III of smCx 24 and 48 hours post-trauma.
Discussion: Taken together, the data revealed significant nerve cell injury without apparent cell death in this model.
Document Type: Research Article
Affiliations: Department of Anatomy and Cell Biology, Scott Hall, Room No. 9312, Wayne State University School of Medicine, 540 E. Canfield, Detroit, MI 48201, USA
Publication date: 2007-06-01
- Authors wishing to cite fast track papers should give the journal name and the article DOI. This will enable reference linking via CrossRef and allow forward and backward citation tracking systems to associate the fast track citation with the final journal reference.
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Information for Advertisers
- Terms & Conditions
- Virtual Maney - Stroke
- Top articles
- Health Sciences Spotlight
- ingentaconnect is not responsible for the content or availability of external websites