IngentaConnect Dendritic cells pulsed with glioma lysates induce immunity agains...

Authors: Pellegatta, S.¹; Poliani, P.L.²; Corno, D.¹; Grisoli, M.³; Cusimano, M.¹; Ubiali, F.⁴; Baggi, F.⁴; Bruzzone, M.G.³; Finocchiaro, G.¹

Source: Neurological Research, Volume 28, Number 5, July 2006 , pp. 527-531(5)

Publisher: Maney Publishing

Abstract:

In recent years, the use of dendritic cells (DC), the most powerful antigen presenting cells, has been proposed for the creation of vaccines against gliomas. This approach has been demonstrated to be safe and non-toxic in phase I or I-II trials (2, 3). Immunotherapy plays a central role in the search for new treatments for glioblastoma multiforme (GBM). In particular, several phase I studies have been performed using DC pulsed by GBM proteins as a vaccine for patients with relapsing GBM. The studies demonstrated that DC vaccination is safe and may produce a significant increase in overall survival. As the first step in the preparation of appropriate conditions for a clinical evaluation in Italy, we have performed pre-clinical experiments on immune-competent mice injected intra-cerebrally with syngeneic GL261GBM cells and treated subcutaneously and intra-tumorally with DC loaded with a GL261 homogenate. These results show that vaccination with DC pulsed with a tumor lysate increases considerably survival in mice bearing intracranial glioblastomas and supports the development of DC-based clinical trials for patients with glioblastomas that do not respond to standard therapies.

Keywords: GLIOMA; DENDRITIC CELLS; IMMUNOTHERAPY

Document Type: Research article

DOI: 10.1179/016164106X116809

Affiliations: 1: Department of Experimental Neuro-Oncology, Istituto Nazionale Neurologico Besta, Milano, Italy 2: Department of Pathology, University of Brescia, Brescia, Italy 3: Department of Radiology, Istituto Nazionale Neurologico Besta, Milano, Italy 4: Department of Neuromuscular Disorders, Istituto Nazionale Neurologico Besta, Milano, Italy

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Dendritic cells pulsed with glioma lysates induce immunity against syngeneic intracranial gliomas and increase survival of tumor-bearing mice