IngentaConnect Co-registration of radiographic and pathologic infarct territory ...

Authors: Komotar, Ricardo J.¹; Mocco, J.¹; Mack, William J.¹; Ransom, Evan R.¹; Zacharia, Brad E.¹; King, Ryan G.¹; Ducruet, Andrew F.¹; Cohen, Hilary G.¹; Arango, Victoria²; Connolly, E. Sander¹

Source: Neurological Research, Volume 27, Number 6, September 2005 , pp. 634-637(4)

Publisher: Maney Publishing

Abstract:

Objectives: Infarct volume correlation using magnetic resonance imaging (MRI) and pathology specimens enables exact tissue localization of cerebral injury following experimental stroke. We describe a protocol that enables co-registration of radiographic signal change and histologic ischemia in a non-human primate model of stroke.

Methods: One male baboon underwent left middle cerebral artery territory occlusion/reperfusion. MRI [5 mm axial T2 weighted (T2W) slices] was carried out 9 days post-ischemia after which the animal was killed. Immediately post-mortem, the whole brain was perfused and fixed in paraformaldehyde and sliced into 5 mm axial sections that corresponded to those demonstrated on MRI. Slices (40 m) were obtained from each section and were then stained using Luxol hematoxylin and eosin.

Results: The relative area of hyperintensity demonstrated on T2W MRI approximates, in size and location, the region of infarct on gross pathology. This was confirmed microscopically.

Discussion: With the use of advanced imaging modalities, this co-registration technique affords the capacity to differentiate ischemic core, penumbra, and uninjured cortex following experimental stroke. Such a precise delineation enables immunohistochemical analysis of a wide variety of substrates in each of the aforementioned regions.

Keywords: STROKE; PRIMATE MODEL; MAGNETIC RESONANCE IMAGING; HISTOLOGY

Document Type: Research article

DOI: 10.1179/016164105X17305

Affiliations: 1: Department of Neurological Surgery, Columbia University, New York, USA 2: Department of Psychiatry, Columbia University, New York, USA

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Co-registration of radiographic and pathologic infarct territory in a non-human primate model of stroke