Authors: Wang, Xiaodan¹; Niwa, Masayuki²; Hara, Akira³; Matsuno, Hiroyuki¹; Kawase, Kazuhide⁴; Kozawa, Osamu¹; Mori, Hideki³; Uematsu, Toshihiko¹

Source: Neurological Research, Volume 24, Number 7, October 2002 , pp. 730-735(6)

Publisher: Maney Publishing

Abstract:

Temporal profile of neuronal deaths in the mouse retina evoked by a transient retinal ischemia and the protective effect of hypothermia on such deaths were evaluated. A transient ischemic insult was induced in the mouse retina by elevating the intra-ocular pressure. The retina tissue responses after reperfusion were histopathologically detected by monitoring the retinal cell death in the ganglion cell layer and inner nuclear layer, using a sequential TUNEL-staining technique, and by measuring the inner retinal thickness. Elevation of intra-ocular pressure induced a time-related appearance of TUNEL-positive cells in the mouse inner retinas. Peak TUNEL staining occurred 12 h after reperfusion. Lowering mice body temperature to 35°C, 33°C and 29°C during the ischemia period significantly inhibited DNA fragmentation of retinal neurons in a lowering temperature dependent manner. In this experiment, the inner retinal thickness was preserved in 29°C group compared with that in 37°C group. From these results, the 45-min transient ischemia and histopathological examination 12 h later provided a reproducible number of retinal neuronal deaths. Furthermore, hypothermic intervention showed a protective effect to salvage retinal neuronal cells from a transient ischemic insult.

Keywords: DEATH; HYPOTHERMIA; NEURONAL; APOPTOSIS; RETINA; ISCHEMIA

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/016164102101200663

Affiliations: 1: Department of Pharmacology, Gifu University School of Medicine, Gifu, Japan 2: Department of Pharmacology and Medical Education Development Center, Gifu University School of Medicine, Gifu, Japan 3: Department of Pathology, Gifu University School of Medicine, Gifu, Japan 4: Department of Ophthalmology, Gifu University School of Medicine, Gifu, Japan

Publication date: 2002-10-01

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