Neuronal degradation in mouse retina after a transient ischemia and protective effect of hypothermia

Authors: Wang X.1; Niwa M.2; Hara A.3; Matsuno H.1; Kawase K.4; Kozawa O.1; Mori H.3; Uematsu T.1

Source: Neurological Research, Volume 24, Number 7, October 2002 , pp. 730-735(6)

Publisher: Maney Publishing

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Abstract:

Temporal profile of neuronal deaths in the mouse retina evoked by a transient retinal ischemia and the protective effect of hypothermia on such deaths were evaluated. A transient ischemic insult was induced in the mouse retina by elevating the intra-ocular pressure. The retina tissue responses after reperfusion were histopathologically detected by monitoring the retinal cell death in the ganglion cell layer and inner nuclear layer, using a sequential TUNEL-staining technique, and by measuring the inner retinal thickness. Elevation of intra-ocular pressure induced a time-related appearance of TUNEL-positive cells in the mouse inner retinas. Peak TUNEL staining occurred 12 h after reperfusion. Lowering mice body temperature to 35°C, 33°C and 29°C during the ischemia period significantly inhibited DNA fragmentation of retinal neurons in a lowering temperature dependent manner. In this experiment, the inner retinal thickness was preserved in 29°C group compared with that in 37°C group. From these results, the 45-min transient ischemia and histopathological examination 12 h later provided a reproducible number of retinal neuronal deaths. Furthermore, hypothermic intervention showed a protective effect to salvage retinal neuronal cells from a transient ischemic insult.

Keywords: RETINA; ISCHEMIA; HYPOTHERMIA; NEURONAL; DEATH; APOPTOSIS

Document Type: Research article

DOI: 10.1179/016164102101200663

Affiliations: 1: Department of Pharmacology, Gifu University School of Medicine, Gifu, Japan 2: Department of Pharmacology and Medical Education Development Center, Gifu University School of Medicine, Gifu, Japan 3: Department of Pathology, Gifu University School of Medicine, Gifu, Japan 4: Department of Ophthalmology, Gifu University School of Medicine, Gifu, Japan

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