Nonenzymatic derived lipid peroxide, 8-iso-PGF2alpha, participates in the pathogenesis of delayed cerebral vasospasm in a canine SAH model

Authors: Sakamoto M.1; Takaki E.1; Yamashita K.1; Watanabe K.1; Tabuchi S.1; Watanabe T.1; Satoh K.2

Source: Neurological Research, Volume 24, Number 3, April 2002 , pp. 301-306(6)

Publisher: Maney Publishing

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Abstract:

We studied whether 8-iso-PGF2alpha, nonenzymatic arachidonyl peroxide, participated in the pathogenesis of delayed vasospasm using a canine subarachnoid hemorrhage (SAH) model. Fourteen adult mongrel dogs were divided into two groups, two-hemorrhage SAH group (n = 8) and control group (n = 6). The contents of 8-iso-PGF 2alpha in CSF, the basilar artery segment, and subarachnoid clot were measured by enzyme immunoassay kit. The CSF 8-iso-PGF2alpha content on Day 7 in the SAH group was 67.9 ± 29.9 pg ml-1 (n = 8), which was significantly higher than 27.1 ± 1 3.8 (n = 8) on Day 0 in the SAH group, and 33.2 ± 14.4 pg ml-1 (n = 5) on Day 7 in the control group. The 8-iso-PGF2alpha content in the basilar artery segment with spasm on Day 7 in the SAH group was 13.5 ± 1.9 pg mg-1 wet weight (n = 8), significantly higher than 8.7 ± 1.9 (n = 6) in the control group. The 8-iso-PGF2alpha content in subarachnoid clot was 1.7 ± 1.4 ng g-1 wet weight (n = 8). Significant elevation of the 8-iso-PGF2alpha contents in the CSF and the basilar artery segment occurred on Day 7 in the SAH group. The subarachnoid clot enclosed the basilar artery on Day 7, contained a considerable amount of 8-iso-PGF2alpha. These results suggested that 8-iso-PGF2alpha could play a crucial role in the pathogenesis of the delayed cerebral vasospasm. [Neurol Res 2002; 24: 301-306]

Keywords: 8-iso-PGF2 ALPHA; VASOSPASM; LIPID PEROXIDATION; NONENZYMATIC; SUBARACHNOID HEMORRHAGE

Document Type: Research article

DOI: 10.1179/016164102101199783

Affiliations: 1: Department of Neurosurgery, Institute of Neurological Sciences, Faculty of Medicine Tottori University, Yonago, Japan 2: Department ofPharmacology, Faculty of Medicine Tottori University, Yonago, Japan

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