Anti-inflammatory effects of daidzein on primary astroglial cell culture

Authors: Liu, Man-Hai¹; Lin, Yu-Shan¹; Sheu, Shiow-Yunn²; Sun, Jui-Sheng³

Source: Nutritional Neuroscience, Volume 12, Number 3, June 2009 , pp. 123-134(12)

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Abstract:

Introduction: Alzheimer's disease is the common cause of dementia in old people. The pathological hallmarks of Alzheimer's disease include neuronal loss, deposition of amyloid-?, and presence of neurofibrillary tangles. The endogenous steroid estrogen has been shown to affect neuronal growth, differentiation and survival, while isoflavones also have a neuroprotective effect on human cortical neurons. Daidzein, however, has a superior neuron-protective effect to other isoflavones. The present study is to determine whether daidzein is able to inhibit the production of pro-inflammatory mediators under amyloid-? and lipopolysaccharide stimulation.

Materials and methods: Astrocyte cells were stimulated with amyloid-? or lipopolysaccharide in the absence and presence of diadzein. Nitric oxide released into the culture media was determined using the Griess reaction, and concentrations of IL-1, IL-6, TNF-? and estrogen receptor gene expression were measured by semi-quantitative real-time polymerase chain reaction assay.

Results: Diadzein-treatment increases astrocyte cell counts and attains its maximal effect at the 10^?12M concentration. The addition of 20 ?M amyloid-? or 10^?6 g/ml LPS can significantly decrease the viability of astrocytes, up-regulated IL-1, IL-6, TNF-? mRNA and estrogen receptor expression; in addition, 1-h daidzein pre-treatment can restore the decreased viability of astrocytes induced by amyloid-? or lipopolysaccharide as well as down-regulate their mRNA expression.

Conclusions: It seems that this response is estrogen receptor-mediated. These results further increase the possibility that daidzein may have potential to ameliorate the inflammatory process and also alleviate the risk of Alzheimer's disease progression.

Keywords: AMYLOID BETA-PEPTIDE; LIPOPOLYSACCHARIDE; DIADZEIN; NEUROPROTECTIVE EFFECT

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/147683009X423274

Affiliations: 1: Graduate Institute of Pharmacy, Taipei Medical University, Taipei, Taiwan, Republic of China 2: School of Pharmacy, Taipei Medical University, Taipei, Taiwan, Republic of China 3: Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan, Republic of China; Department of Orthopedic Surgery, National Taiwan University Hospital, Taipei, Taiwan, Republic of China

Publication date: 2009-06-01

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Anti-inflammatory effects of daidzein on primary astroglial cell culture

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