GM1 and NGF modulate Ca²⁺ homeostasis and GAP43 mRNA expression in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate

Authors: Huang, Fei¹; Liu, Zhen²; Liu, Huaxiang³; Wang, Lihong²; Wang, Huaijing²; Li, Zhenzhong²

Source: Nutritional Neuroscience, Volume 10, Numbers 3-4, June/August 2007 , pp. 105-111(7)

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Abstract:

Monosialoganglioside (GM1) has been considered to have a neurotrophic factor-like activity. Nerve growth factor (NGF), a member of the neurotrophin family, is essential for neuronal survival, differentiation and maturation. The aim of the present study was to investigate whether co-administration of GM1 and NGF reverses glutamate (Glu) neurotoxicity in primary cultured rat embryonic dorsal root ganglion (DRG) neurons. DRG neurons were exposed to Glu (2 mmol/l), Glu (2 mmol/l) plus GM1 (10 μg/ml), Glu (2 mmol/l) plus NGF (10 ng/ml), Glu (2 mmol/l) plus GM1 (5 μg/ml) and NGF (5 ng/ml) and then processed for detecting intracellular concentrations of Ca²⁺ ([Ca²⁻]_i) by confocal laser scanning microscopy and growthassociated protein 43 (GAP43) mRNA by RT-PCR. The fluorescent intensity in Glu plus GM1 and NGF incubated neurons was the lowest as compared with that in other groups. The expression of GAP43 mRNA in Glu plus GM1 and NGF incubated neurons was the highest as compared with that in other groups. These results implicated that GM1 and NGF have synergistic neuroprotective effects on DRG neurons with excitotoxicity induced by Glu in vitro.

Keywords: DORSAL ROOT GANGLION; NEUROTOXICITY; NEUROPROTECTIVE EFFECT; GLUTAMATE; NGF; GM1

Document Type: Research Article

DOI: http://dx.doi.org/10.1080/10284150701406752

Affiliations: 1: Department of Anatomy, Shandong University School of Medicine, Jinan 250012, People's Republic of China; Department of Anatomy, Binzhou Medical College, Binzhou 256603, People's Republic of China 2: Department of Anatomy, Shandong University School of Medicine, Jinan 250012, People's Republic of China 3: Department of Rheumatology, Shandong University Qilu Hospital, Jinan 250012, People's Republic of China

Publication date: 2007-06-01

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