Authors: Buczkowska, Katarzyna¹; Dabert, Miroslawa²

Source: Journal of Bryology, Volume 33, Number 4, 2011 , pp. 291-299(9)

Publisher: Maney Publishing

Abstract:

The genus Calypogeia belongs to one of the most difficult groups of leafy liverworts, with about 90 species. Nine species of the genus are currently known from Europe: C. azurea, C. integristipula, C. neesiana, C. suecica, C. muelleriana, C. sphagnicola, C. fissa, C. arguta, and C. azorica. Recently, another species, morphologically resembling C. muelleriana but genetically distinct from it, was detected using isozyme markers. In the present study, C. muelleriana and C. sp. nov. were further characterized by 13 and 14 specific inter-simple sequence repeat band patterns, respectively. Nei’s genetic distance between the two species was 0·61. Three species-specific bands were converted into sequence characterized amplified region (SCAR) markers. The first SCAR primer pair amplified a product of 360 and 600 bp in C. muelleriana and C. sp. nov., respectively. The two other SCAR markers were diagnostic for other European Calypogeia species.

Keywords: Liverworts; ISSR; SCAR; Calypogeia

Document Type: Original Article

DOI: http://dx.doi.org/10.1179/1743282011Y.0000000029

Affiliations: 1: Department of Genetics, Institute of Experimental Biology 2: Molecular Biology Techniques Laboratory, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

Publication date: 2011-12-01

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• The complete content of Transactions of the British Bryological Society, the predecessor to Journal of Bryology, going back to 1947, is also available online. View Transactions of the British Bryological Society issues.
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