Giemsa Staining of Tissue Sections: A New Twist on an Old Technique

Author: Buesa, Rene J.

Source: Journal of Histotechnology, Number 4, December 2001 , pp. 251-253(3)

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Abstract:

The described procedure results in excellent staining and differentiation of all tissue components with straightforward steps. Slides are immersed in the Giemsa working solution, prepared using a commercial stock solution and tris-maleic buffer, heated to ≈60°C in a microwave oven, and stained for 10 minutes at room temperature. Differentiation is obtained with a 1% acetic acid solution in absolute ethanol, which also initiates the dehydration process. The slides are differentiated one at a time until no more blue color flows from the sections. Dehydration and clearing are completed quickly, and the slides are coverslipped as usual. From the initial dewaxing, the whole procedure is completed in less than 30 min. The stain does not fade with time. (The J Histotechnol 24:251, 2001)

Submitted: December 8, 2000; Accepted March 22, 2001

Keywords: microwave irradiation; Tris-maleic buffer; acetic acid differentiation

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/014788801794812273

Publication date: 2001-12-01

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