A Modified Sulfation-Toluidine Blue Technique for the Demonstration of Fungi in Tissue Sections
Authors: Smith, Danny J.; Lowrey, Terry
Source: Journal of Histotechnology, Number 1, March 1986 , pp. 23-24(2)
Publisher: Maney Publishing
Abstract:A modification of the sulfation-toluidine blue procedure for the detection of fungi in tissue is described. This method uses a sulfation reagent, which is safer to mix and use than the previous reagent, and a counterstain, which provides an improved contrast between the fungi and the background. This modification method is simple to use, and the results are comparable to the original stain.
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