Optimal Conditions for Demonstrating Human Lymphocyte Differentiation Antigens: A Working Protocol for the Immunohistochemical Analysis of a Lymph Node Biopsy

Authors: Elias, Jules M.; La Neve, Debra; Beutner, Ernst H.; Hogrefe, Wayne; Kately, John; Britten, Tom; Rousso, Carmen

Source: Journal of Histotechnology, Number 4, December 1985 , pp. 201-203(3)

Publisher: Maney Publishing

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Abstract:

The histologic requirements for preservation of lymphocyte membrane antigens were discussed by the participants of a conference on leukemia and lymphoma. The risk of false negative staining is minimized if surgical lymph node biopsy specimens are processed promptly into OCT and quick-frozen with liquid nitrogen. Cut sections must be adequately dried and fixed in “dry” acetone.

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/014788885794594580

Publication date: 1985-12-01

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