Fluorescent Thiazole Stains for Amyloid Without Differentiation

Authors: Waldrop, F. S.; Puchtler, H.; Meloan, S. N.

Source: Journal of Histotechnology, Number 3, September 1984 , pp. 123-126(4)

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Abstract:

Conventional Thioflavine T and S stains for fluorescence microscopy of amyloid require differentiation and are not specific. The selective fluorescent brightening agent Phorwhite BBU is currently unavailable on the biological dye market. We therefore searched for other methods. Histochemical studies indicated similarities in the binding of benzidine and thiazole dyes by amyloid. Three direct and two cationic thiazole dyes were tested further under the conditions of the alkaline Congo red and the Phorwhite BBU reaction and proved suitable for demonstration of amyloid and paramyloid. In two cases amyloid deposits stained with cationic thiazole dyes showed differential fluorescence. The staining properties of different batches of the same dye can vary drastically; this heterogeneity of thiazole dyes is inherent in their mode of synthesis. Therefore, thiazole dyes should be regarded only as a stop-gap until selective modern fluorescent brightening agents become available again to histologists.

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/014788884794595193

Publication date: 1984-09-01

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