Freeze-Substituted Substrate for ANA Detection
Authors: Elias, J. M.; Halpryn, B.
Source: Journal of Histotechnology, Number 1, March 1979 , pp. 27-28(2)
Publisher: Maney Publishing
Abstract:Immunofluorescent anti-nuclear antibody tests combined with chessboard titration studies were used to compare tissue reactivity of standard frozen cryostat sections and freeze substituted tissues. Frozen sections of rat kidney were slightly more sensitive than freeze-substituted sections, but nuclear diffuse and speckled staining patterns were more easily discerned in the freeze-substituted tissues due to overall better preservation of tissue morphology.
Document Type: Research Article
Publication date: 1979-03-01
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