Homing and engraftment of bone marrow cells derived from different donors in a murine model of sensitization

Authors: Xu, Lu-Hong; Fang, Jian-Pei; Xu, Hong-Gui; Weng, Wen-Jun

Source: Hematology, Volume 17, Number 4, July 2012 , pp. 215-219(5)

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Abstract:

Sensitized recipients are at a high risk of graft rejection in hematopoietic stem cell transplantation. To explore the trace of donor cells, we tried to explore homing and engraftment of bone marrow cells (BMCs) derived from different donors in a murine model of sensitization. Sensitized BALB/c mice were used as transplanted recipients, which received BMCs derived from C57BL/6 or BALB/c donors after lethal irradiation. The homing study showed that the donor cells decreased along with time in recipients of the C57BL/6 donor group, but the donor cells increased along with time in recipients of the BALB/c donor group. For the engraftment assay, all the sensitized recipients transplanted with BMCs derived from C57BL/6 donors died after lethal irradiation. In contrast, all the recipients transplanted with BMCs derived from BALB/c donors got long-term survival. Our results suggest that it is crucial to have human leukocyte antigen identical donors for sensitized recipients during hematopoietic stem cell transplantation.

Keywords: Homing; Engraftment; Bone marrow cells; Sensitization

Document Type: Research article

DOI: http://dx.doi.org/10.1179/102453312X13376952196539

Affiliations: 1: Department of Pediatrics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China; and Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, Sun Yat-sen University, Guangzho

Publication date: 2012-07-01

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