Authors: Gutiérrez-Cisneros, M. J.¹; Cogollos, R.²; López-Vélez, R.³; Martín-Rabadán, P.⁴; Martínez-Ruiz, R.⁵; Subirats, M.⁶; Merino, F. J.⁷; Fuentes, I.¹

Source: Annals of Tropical Medicine and Parasitology, Volume 104, Number 2, March 2010 , pp. 145-149(5)

Publisher: Maney Publishing

Abstract:

In an effort to improve the diagnosis of intestinal amoebiasis, a real-time PCR has been used for the detection and differentiation of Entamoeba histolytica and E. dispar infections in African or South American immigrants who live in Spain. Faecal samples from all of the 130 subjects had apparently been found to contain E. histolytica/E. dispar cysts by microscopical examination. Using the real-time PCR, E. histolytica DNA was detected in faecal samples from only 10 (7.7%) of the immigrants, with E. dispar DNA detected in the samples from another 117 (90.0%) of the subjects. The use of such PCR in the routine investigation of patients found positive for E. histolytica/E. dispar cysts (by microscopy) is recommended, especially in non-endemic areas.

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/136485910X12607012373759

Affiliations: 1: Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera Pozuelo–Majadahonda Km-2, 28220, Madrid, Spain 2: Servicio de Microbiología, Hospital de Móstoles, Río Júcar s/n, 28935, Móstoles, Madrid, Spain 3: Medicina Tropical y Parasitología Clínica, Servicio de Enfermedades Infecciosas, Hospital Ramón y Cajal, Carretera de Colmenar Km 9.1, 28034, Madrid, Spain 4: Servicio de Microbiología, Hospital Gregorio Maranñón, Calle Doctor Esquerdo 46, 28007, Madrid, Spain 5: Servicio de Microbiología, Hospital Puerta de Hierro, Joaquín Rodrigo 2, 28222, Majadahonda, Madrid, Spain 6: Servicio de Microbiología, Hospital Carlos III, Calle de Sinesio Delgado 10, 28029, Madrid, Spain 7: Servicio de Microbiología, Hospital Severo Ochoa, Avenida de Orellana s/n, 28911, Leganés, Madrid, Spain

Publication date: 2010-03-01

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• In 2012 Annals of Tropical Medicine and Parasitology changed its name to Pathogens of Global Health to reflect changes and developments in the subject area. View the issues of Pathogens of Global Health available online..
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