Identification of the 18S-ribosomal-DNA genotypes of Acanthamoeba isolates from the Philippines
Authors: Rivera, W.L.1; Adao, D.E.V.1
Source: Annals of Tropical Medicine and Parasitology, Volume 102, Number 8, December 2008 , pp. 671-677(7)
Publisher: Maney Publishing
- Free Content
- New Content
- Subscribed Content
- Free Trial Content
Abstract:
Cyst morphology has been commonly used to identify the free-living amoeba Acanthamoeba to subgenus level. A more accurate and consistent method, based on the sequence analysis of the gene coding for the amoeba's small-subunit ribosomal RNA (Rns), has, however, been developed. There have been no attempts to identify the Acanthamoeba genotypes circulating in the Philippines. In this study, therefore, the ASA.S1 region of the Rns gene from 17 Acanthamoeba isolates, collected from soil, water and contact-lens storage cases in different regions of the Philippines, was sequenced. After the isolates were genotyped, using the BLAST program, their phylogenetic positions relative to known Acanthamoeba isolates were determined. For this, the model-based (GTR + Γ) neighbour-joining, maximum-likelihood and Bayesian-inference analyses and the non-model-based maximum-parsimony analysis were used. All but two of the isolates were identified as the T5 or T4 genotypes, which are probably common in soil, water and contact-lens cases across the Philippines. The only other genotypes identified were T15 (as a single isolate from a contact-lens case) and T3 (as a single soil isolate).Document Type: Research article
DOI: 10.1179/136485908X337544
Affiliations: 1: Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines; Molecular Protozoology Laboratory, Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City 1101, Phi
- Free Content
- New Content
- Subscribed Content
- Free Trial Content
Click here for Page Help