Chronic American trypanosomiasis: parasite persistence in endomyocardial biopsies is associated with high-grade myocarditis
Authors: Benvenuti, L. A.; Roggério, A.; Freitas, H. F. G.; Mansur, A. J.; Fiorelli, A.; Higuchi, M. L.
Source: Annals of Tropical Medicine and Parasitology, Volume 102, Number 6, September 2008 , pp. 481-487(7)
Publisher: Maney Publishing
Abstract:Myocyte diameter, fractional area of collagen, intensity of myocarditis and parasite persistence (explored by immunohistochemistry and PCR) were evaluated in serial sections of endomyocardial biopsies from 29 outpatients with chronic chagasic cardiopathy. The patients, 25 males and four females with a mean (S.D.) age of 43 (9) years, were subsequently followed up for 3–2861 days (median=369 days). During this follow-up, 16 (55%) of the patients died.
The biopsies revealed myocarditis in 25 (86%) of the patients and high-grade myocarditis in 14 (56%). Although immunohistochemistry failed to demonstrate Trypanosoma cruzi antigens in any of the samples, five (33%) of the 15 biopsies successfully tested in the PCR-based assay for T. cruzi DNA were found positive, indicating parasite persistence. There was a significant positive association between myocardial parasite persistence and high-grade myocarditis (P=0.014); five (71%) of the seven endomyocardial biopsies with high-grade myocarditis that were successfully tested in the PCR assays showed persistent T. cruzi DNA. The survival time of the patients was not, however, found to be significantly associated with myocardial parasite persistence, any of the morphometric measurements taken, or the presence or intensity of myocarditis.
Document Type: Research Article
Affiliations: Heart Institute (InCor), University of São Paulo Medical School, Avenida Dr Enéas de Carvalho Aguiar 44, 05403-000 São Paulo, Brazil
Publication date: 2008-09-01
- In 2012 Annals of Tropical Medicine and Parasitology changed its name to Pathogens and Global Health to reflect changes and developments in the subject area. View the issues of Pathogens and Global Health available online..
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Information for Advertisers
- Terms & Conditions
- Top articles
- Health Sciences Spotlight
- Abstracted in
- ingentaconnect is not responsible for the content or availability of external websites