Microculture for the isolation of Leishmania parasites from cutaneous lesions — Sri Lankan experience

Authors: Ihalamulla, R.L.¹; Rajapaksa, U.S.¹; Karunaweera, N.D.¹

Source: Annals of Tropical Medicine and Parasitology, Volume 99, Number 6, September 2005 , pp. 571-575(5)

Publisher: Maney Publishing

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Abstract:

Novy, McNeal and Nicolle (NNN) medium and Evans' modified Tobie's medium are two conventional media for the isolation of Leishmania parasites in in-vitro cultures. Both are biphasic, with a solid layer of blood agar, and are normally prepared in glass test-tubes. In Sri Lanka at least, a monophasic microcapillary culture, based solely on RPMI 1640 medium supplemented with foetal calf serum, has been found simpler, more economical and more sensitive, for the isolation of L. donovani from skin lesions, than the use of Evans' modified Tobie's medium.

Document Type: Research article

DOI: 10.1179/136485905X51364

Affiliations: 1: Department of Parasitology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo 08, Sri Lanka

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