@article {Leelayoova:1 December 2002:0003-4983:803,
author = "Leelayoova S.",
author = "Taamasri P.",
author = "Rangsin R.",
author = "Naaglor T.",
author = "Thathaisong U.",
author = "Mungthin M.",
title = "In-vitro cultivation: a sensitive method for detecting Blastocystis hominis",
journal = "Annals of Tropical Medicine and Parasitology",
volume = "96",
year = "1 December 2002",
abstract = "Currently, the detection of human infection with Blastocystis hominis is usually based on the examination under a light microscope of faecal samples, either directly, as 'simple smears', or after some form of concentration. Whether short-term, in-vitro cultivation would increase the sensitivity of such detection remains a matter of controversy. Over 900 fresh stool specimens, from soldiers in the Royal Thai Army, were each checked for the parasite using three methods: simple smears; formalin-ethyl-acetate concentration; and cultivation in Jones' medium. Although 334 of the samples were found to be culture-positive, the parasites were only detected in 142 of the simple smears, and faecal concentration led to an even lower sensitivity (64 positive samples). In-vitro cultivation does seem worthwhile in the detection of B. hominis carriage in field studies.",
pages = "803-807(5)",
url = "http://www.ingentaconnect.com/content/maney/atmp/2002/00000096/00000008/art00007"
doi = "doi:10.1179/000349802125002275"
}