The humoral immune responses elicited in mice by inoculations with a recombinant protein or DNA based on the circumsporozoite-protein gene of Plasmodium falciparum
Authors: Fang, Z.; Liu, Y.-W.; Shi, Y.-K.; Yu, X.-B.; Huang, W.-Q.; Ji, X.
Source: Annals of Tropical Medicine and Parasitology, Volume 96, Number 5, July 2002 , pp. 463-468(6)
Publisher: Maney Publishing
Abstract:The humoral responses elicited in mice by inoculation, in various doses and by several routes, with plasmid DNA containing the gene coding for the circumsporozoite protein (CSP) of Plasmodium falciparum FCC1/HN were compared with those evoked by inoculation with a recombinant expressed protein based on the CSP. With the DNA vaccine, intramuscular inoculations appeared the most effective, followed by intravenous and then subcutaneous injections, the responses in each case being dose-dependent. In both standard ELISA and dot-ELISA, sera from the mice immunized with the DNA were found to have much lower titres of antimalarial antibodies than the corresponding sera from mice immunized with the recombinant protein. Although both 'vaccines' elicited humoral immune responses in BALB/c mice, that based on plasmid DNA took much longer than the recombinant protein to induce high-titre antibody responses.
Document Type: Original Article
Affiliations: Department of Parasitology, Nantong Medical College, Nantong, Jiangsu, 226001, China
Publication date: July 2002
- In 2012 Annals of Tropical Medicine and Parasitology changed its name to Pathogens and Global Health to reflect changes and developments in the subject area. View the issues of Pathogens and Global Health available online..
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