Authors: Matar, G. M.¹; Abdo, D.¹; Khneisser, I.¹; Youssef, M.²; Zouheiry, H.³; Abdelnour, G.⁴; Harakeh, H. S.⁵

Source: Annals of Tropical Medicine and Parasitology, Volume 96, Number 3, April 2002 , pp. 317-324(8)

Publisher: Maney Publishing

Abstract:

In several hospitals in Beirut, Lebanon, 77 isolates of Escherichia coli were successfully derived from the stools of patients with diarrhoeal diseases, by culture on MacConkey or MacConkey-sorbitol agar. When the isolates were screened, using a multiplex PCR, 14 (from 14 different patients) were each found positive for one of the various genes defining the enterotoxigenic (five), enteroinvasive (four), enteroaggregative (three) or enteropathogenic (two) groups. Genotyping of these 14 diarrhoeagenic isolates, by pulsed-field gel electrophoresis, indicated that all were genomically distinct with the exception of two of the enteroaggregative isolates (which were of the same genotype). The E. coli apparently involved in diarrhoeal disease in Beirut therefore belong to at least four different diarrhoeagenic groups and show strain variation within each group. Diarrhoea in the absence of diarrhoeagenic E. coli may be the result of infection with bacteria other than E. coli or viral or parasitic enteropathogens.

Keywords:

Document Type: Research Article

DOI: http://dx.doi.org/10.1179/000349802125001032

Affiliations: 1: Department of Microbiology and Immunology, American University of Beirut, P.O. Box 11-0236, Riad El-Solh, Beirut, Lebanon 2: Department of Biological Sciences, Yarmouk University, Irbid, Jordan 3: Lebanese University, P.O. Box 14-6573, Place du Musée, Beirut, Lebanon 4: Haigazian College, P.O. Box 11-1748, Riad El-Solh, Beirut, Lebanon 5: Department of Biology, American University of Beirut, P.O. Box 11-0236, Riad El-Solh, Beirut, Lebanon

Publication date: 2002-04-01

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• In 2012 Annals of Tropical Medicine and Parasitology changed its name to Pathogens and Global Health to reflect changes and developments in the subject area. View the issues of Pathogens and Global Health available online..
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