Tobacco Transformants Bearing Antisense Suppressor of Proline Dehydrogenase Gene, Are Characterized by Higher Proline Content and Cytoplasm Osmotic Pressure

Authors: Kochetov A.V.¹; Titov S.E.²; Kolodyazhnaya Y.S.²; Komarova M.L.²; Koval V.S.²; Makarova N.N.²; Il'yinskyi Y.Y.²; Trifonova E.A.²; Shumny V.K.¹

Source: Russian Journal of Genetics, Volume 40, Number 2, February 2004 , pp. 216-218(3)

Publisher: MAIK Nauka/Interperiodica

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Abstract:

The antisense suppressor was constructed for proline dehydrogenase gene (PDH; a fragment of PDH from Arabidopsis in antisense orientation and under the control of 35S promoter of cauliflower mosaic virus, CMV). In Nicotiana tabacum SR1 tobacco transformants bearing antisense suppressor for PDH, the proline content and the cytoplasm osmotic pressure were increased. The proline content in these transformants varied, whereas cytoplasm osmotic pressure was stable, which seems to reflect complicated relationships between these characteristics of the plant cell.

Document Type: Research article

DOI: http://dx.doi.org/10.1023/B:RUGE.0000016999.53466.e1

Affiliations: 1: Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia ak@bionet.nsc.ru. Novosibirsk State University, Novosibirsk, 630090 Russia, Email: ak@bionet.nsc.ru 2: Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia ak@bionet.nsc.ru, Email: ak@bionet.nsc.ru

Publication date: 2004-02-01