Effect of Synthetic -Endorphin-Like Peptide Immunorphin on Human T Lymphocytes

Authors: Navolotskaya, E.V.1; Malkova, N.V.2; Zargarova, T.A.2; Lepikhova, T.N.2; Krasnova, S.B.2; Lipkin, V.M.2

Source: Biochemistry (Moscow), 1 March 2002, vol. 67, no. 3, pp. 357-363(7)

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Abstract:

-Endorphin and the synthetic -endorphin-like decapeptide Ser-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr (referred to as immunorphin), corresponding to the sequence 364-373 of the CH3 domain of human immunoglobulin G heavy chain, were shown to stimulate concanavalin A-induced proliferation of T lymphocytes from the blood of healthy donors. [Met^5]Enkephalin and the antagonist of opioid receptors naloxone examined in parallel were inactive. The stimulating effect of -endorphin and immunorphin on T lymphocyte proliferation is not inhibited by naloxone. Studies on receptor binding of ^125I-labeled immunorphin to T lymphocytes revealed that it binds with high affinity to naloxone-insensitive receptors (K_d = 7.0 ± 0.3 nM)). Unlabeled immunorphin completely inhibits ^125I-labeled -endorphin specific binding to naloxone insensitive receptors on T lymphocytes (K_i = 0.6 ± 0.1 nM)). Thus, -endorphin and immunorphin interact with common naloxone insensitive receptors on T lymphocytes.

Keywords: -endorphin; T lymphocytes; immunoglobulin G (IgG); naloxone; peptides; receptors

Document Type: Regular Paper

Affiliations: 1: Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow Region, 142290 Russia navolots@fibkh.serpukhov.su 2: Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow Region, 142290 Russia

Publication date: March 1, 2002

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