Water-Soluble Galactomannan from the Seed of Ground Honeysuckle (Lotus corniculatus L.): Structure and Properties

Authors: Egorov A.V.1; Mestechkina N.M.1; Plennik R.Y.2; Shcherbukhin V.D.1

Source: Applied Biochemistry and Microbiology, Volume 39, Number 5, September 2003 , pp. 509-511(3)

Publisher: MAIK Nauka/Interperiodica

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Abstract:

Galactomannan, a water-soluble heteropolysaccharide, was isolated from the seed of a Far Eastern population of the ground honeysuckle Lotus corniculatus L. (yield, 1.65%). Analysis of this galactomannan showed that it consists of D-mannose and D-galactose residues (molar ratio, 1.22 : 1). Its aqueous solutions were characterized by a specific rotation [alpha]D = +84.1° and intrinsic viscosity [eta] = 559 ml/g. Analysis of this heteropolysaccharide using chemical and enzymatic procedures, as well as IR and 13C NMR spectroscopy, showed that its main chain comprises 1,4-beta-D-mannopyranose residues, 95.5% of which are substituted at C-6 with single residues of alpha-D-galactopyranose.

Language: English

Document Type: Research article

Affiliations: 1: Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071 Russia vds@inbi.ras.ru 2: Central Siberian Botanical Garden, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630090 Russia

Publication date: 2003-09-01

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