A New Method for Differential Quantitative Assay of Zeatin and Zeatin Riboside

Authors: Blintsov A.N.¹; Gusakovskaya M.A.¹; Ermakov I.P.¹

Source: Applied Biochemistry and Microbiology, Volume 37, Number 4, 8 July 2001 , pp. 425-429(5)

Publisher: MAIK Nauka/Interperiodica

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In this Subject: Anatomy & Physiology , Organic Chemistry , Biochemistry
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Abstract:

A new method is proposed for differential quantitative assay of two major endogenous cytokinin forms. It is based on determination of two effective parameters—concentrations of zeatin and zeatin riboside—with the use of appropriate antigens as standards. The method can be used for determining cytokinins in small samples of plant tissues without extract fractionation. This study pioneers the quantitation of changes in the hormonal status of ovules and ovaries of Triticum aestivumL. at early stages of embryogeny. A gradual increase in the content of the active and storage forms of the hormones from the ovary to the ovule was revealed.

Language: English

Document Type: Regular paper

Affiliations: 1: Moscow State University, Vorob'evy gory, Moscow, 119899 Russia