Solid-state fermentation production of tetracycline by Streptomyces strains using some agricultural wastes as substrate

Authors: Agnes Asagbra¹; Abiodun Sanni²; Olusola Oyewole³

Source: World Journal of Microbiology and Biotechnology, Volume 21, Number 2, March 2005 , pp. 107-114(8)

Publisher: Springer

Buy & download fulltext article:

Price: $47.00 plus tax (Refund Policy)

Abstract:

The ability of Streptomyces sp. OXCI, S. rimosus NRRL B2659, S. rimosus NRRL B2234, S. alboflavus NRRL B1273 S. aureofaciens NRRL B2183 and S. vendagensis ATCC 25507 to produce tetracycline using some local agricultural wastes as solid state media, were assessed. The wastes employed include peanut (groundnut) shells, corncob, corn pomace and cassava peels. Bacillus subtilis ATCC 6633 was used to assay antimicrobial activity. All the strains produced tetracycline in a solid-state fermentation process containing peanut (groundnut) as the carbohydrate source. Streptomyces sp. OXC1 had the highest ability for tetracycline production with peanut shells as the substrate in solid fermentation (13.18 mg/g), followed by S. vendagensis ATCC 25507 (11.08 mg/g), S.␣rimosus NRRL B1679 (8.46 mg/g), S. alboflavus NRRL B1273 (7.59 mg/g), S. rimosus NRRL B2234 (6.37 mg/g), S. aureofaciens NRRL B2183 (4.27 mg/g). Peanut (groundnut) shells were the most effective substrate (4.36 mg/g) followed by corncob (2.64 mg/g), cassava peels (2.16 mg/g) and corn pomace (1.99 mg/g). The composition of the peanut (groundnut) shell medium optimal for tetracycline production were peanut shells 100 g, organic nitrogen (peanut meal) 10 g, (NH ₄)₂ SO₄ 1 g, KH₂ PO₄ 0.5 g, CaCO₃ > 0.5 g, NaCl 0.5 g, MgSO₄ · 7H₂ O 0.5 g, soluble starch 10 g, peanut oil 0.25 ml with initial moisture content of 65–68%, and initial pH 5.3–6.3. Substrate (1 g dry weight) was inoculated with 1.0 × 10 ⁸ conidia per ml and incubated at 28–31 °C for 5–7 days, producing 13.18 mg/g of total tetracycline. Tetracycline detection started on day 3 and attained its maximum level on day 5.

Keywords: Optimisation; peanut shells; solid-state fermentation; Streptomyces; tetracycline

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s11274-004-2778-z

Affiliations: 1: Biotechnology Division, Federal Institute of Industrial Research, Oshodi, Nigeria, Email: yemasagbra@yahoo.com 2: Department of Botany and Microbiology, University of Ibadan, Nigeria, 3: Department of Food Science and Technology, University of Agriculture Abeokuta, Nigeria,

Publication date: 2005-03-01

Solid-state fermentation production of tetracycline by Streptomyces strains using some agricultural wastes as substrate

Buy & download fulltext article:

Tools

Key

Text size:

Useful pages