Short Communication: Cloning of genes involved in the antifungalactivity of a fluorescent Pseudomonas sp.
Authors: Anith K.N.1; Tilak K.V.B.R.2; Khanuja S.P.S.3; Saxena A.K.2
Source: World Journal of Microbiology and Biotechnology, Volume 14, Number 6, November 1998 , pp. 939-941(3)
Publisher: Springer
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Abstract:
The molecular basis of antifungal toxin production by a fluorescent Pseudomonas strain EM 85 was studied. The bacterial strain showed in vitro inhibition of growth of Rhizoctonia solani. NTG mutagenesis of the wild type strain helped in the isolation of a antifungal-toxin-defective mutant AN 21. A genomic library of the wild type strain was constructed in the cosmid vector pLAFR 1 and maintained in an E. coli background. Complementation analysis with cosmid library resulted in the isolation of a cosmid clone which complemented the defective character in the mutant AN 21. The size of the complementing DNA fragment was found to be 23.5kb.
Keywords: Antifungal toxin; biological control; Pseudomonads
Language: English
Document Type: Regular paper
Affiliations: 1: Plant Biotechnology and Molecular Biology Centre, Regional Agricultural Research Station, Ambalavayal, Wayanad, Kerala, India; Tel.: (0493) 660561, 660421. 2: Division of Microbiology, Indian Agricultural Research Institute, New Delhi, India. 3: Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
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