Relationship Between Sulphate Starvation and Chromate Reduction in a H₂-fed Fixed-film Bioreactor

Authors: Battaglia-Brunet, F.; Michel, C.; Joulian, C.; Ollivier, B.; Ignatiadis, I.

Source: Water, Air, and Soil Pollution, Volume 183, Numbers 1-4, July 2007 , pp. 341-353(13)

Publisher: Springer

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Abstract:

While developing a low-sulphate system combining indirect chromate-reduction by biologically-produced hydrogen sulphide and direct biological chromate-reduction to treat chromate-bearing waters, the aim of the present work was to evaluate the influence of sulphate and H₂ starvation on chromate reduction. Chromate-reduction was performed under continuous-feed conditions in a fixed-film column bioreactor originally inoculated with a bacterial consortium containing Desulfomicrobium norvegicum, and fed with H₂. With 500 mg l⁻¹ of sulphate in the feed solution, total chromate-reduction was observed in the effluent whereas sulphate-reduction was strongly decreased, as also confirmed by measurements of isotopic ratios for sulphur. In the absence of sulphate, a chromate-reduction activity was still observed but was lower than in the presence of sulphate, and chromate-reduction was H₂-dependent. Molecular biology techniques revealed the composition of the bacterial population in the effluent. D. norvegicum together with other micro-organisms of the Bacteria domain were detected. They include members related to the genera Acinetobacter, Acetobacterium and Rhodocyclus. Even when sulphate-reduction was strongly decreased, the presence of sulphate enhances the efficiency of the H₂-dependent chromate-reduction. A H₂- and CO₂-consuming bacterial population may be used in a globally autotrophic process to reduce chromate at low sulphate concentration, thus avoiding excess sulphide production.

Keywords: Bioreactor; Chromate-reduction; Desulfomicrobium norvegicum; Fixed-film; Hydrogen; Molecular ecology; Sulphate-reduction; 16S rRNA gene; dsrAB gene

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s11270-007-9383-3

Affiliations: 1: Email: i.ignatiadis@brgm.fr

Publication date: 2007-07-01