Site of Interaction of Copper on Bacillus Polymyxa
Authors: Philip L.1; Iyengar L.2; Venkobachar C.3
Source: Water, Air, and Soil Pollution, Volume 119, Numbers 1-4, April 2000 , pp. 11-21(11)
Publisher: Springer
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Abstract:
Copper uptake potential of different bacterial strains isolated from contaminated soil was compared. The bacterial strain identified as Bacillus polymyxa showed maximum copper uptake capacity. An attempt was made to elucidate the site of interaction of Cu(II) with B. polymyxa using electron paramagnetic resonance (EPR) studies. The EPR spectra showed that accumulated copper formed complexes with oxygen and nitrogen which may be the carboxyl groups of cell-wall peptidoglycan and nitrogen atoms of aminosugars or structural proteins. The absence of super hyperfine splitting in the g 
region of EPR spectra of treated B. polymyxa cells after Cu(II) uptake indicated that copper could be coordinated with the oxygen atoms from carboxyl groups of peptidoglycan in the cell walls.
Keywords: Bacillus polymyxa; biosorption; copper; EPR spectra; site of interaction
Language: English
Document Type: Regular paper
Affiliations: 1: Dept. of Civil Engineering, Indian Institute of Technology, Kharagpur, India-721 302 (author for correspondence, e-mail: ligy@civil.iitkgp.ernet.in) 2: Dept. of Chemistry, Indian Institute of Technology, Kanpur, India 3: Dept. of Civil Engineering, University of West Indies, St. Augustine, Trinidad, WI
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