The sensitivity of embryogenic tissue of Picea omorika (Pan.) Purk. to antibiotics

Authors: Mihaljevi S.¹; Peri M.²; Jelaska S.³

Source: Plant Cell, Tissue and Organ Culture, Volume 67, Number 3, December 2001 , pp. 287-293(7)

Publisher: Springer

Abstract:

In an attempt to develop a transformation procedure for somatic embryos of Picea omorika (Pan.) Purk. using Agrobacterium, the sensitivity of non-transformed embryogenic tissues of P. omorika to antibiotics was measured. Two groups of antibiotics were tested: antibiotics commonly used to eliminate Agrobacterium from tissue culture (carbenicillin and cefotaxime), and antibiotics for the selection of transformed tissue (kanamycin, paromomycin, neomycin and hygromycin). Carbenicillin (500 mg l^−1) reduced proliferation of P. omorika embryogenic tissue by 50% as compared to the control. Cefotaxime (500 mg l^−1) was less toxic to embryogenic tissue growth (93% of the control) and is thus more suitable for the elimination of A. tumefaciens from embryogenic tissue of P. omorika. Embryogenic tissue showed severe susceptibility to kanamycin and hygromycin. Induction of secondary embryogenesis was blocked by 10 mg l^−1 of kanamycin or 2 mg l^−1 of hygromycin. The aminoglycoside analogs of kanamycin, paromomycin and neomycin inhibited embryogenic tissue induction at concentrations of 5 and 100 mg l^−1, respectively. The higher tolerance of embryogenic tissue to neomycin indicated that of the antibiotics tested neomycin may be most useful for selection of nptII-transformed embryogenic tissue of Picea omorika.

Keywords: cefotaxime; kanamycin; neomycin; omorika spruce; paromomycin

Language: English

Document Type: Regular paper

Affiliations: 1: Institute "Ruder Bokovi", P. O. BOX 180, HR-10002 Zagreb, Croatia; requests for offprints; Fax: +385-1456-0177; E-mail: mihaljev@rudjer.irb.hr) kovi", P. O. BOX 180, HR-10002 Zagreb, Croatia; requests for offprints; Fax: +385-1456-0177; E-mail: mihaljev@rudjer.irb.hr) "> 2: University of Zagreb, Faculty of Science, Department of Molecular Biology, Rooseveltov trg 6, HR-10000 Zagreb, Croatia; present address: Pliva, Research and Development, Prilaz b. Filipovia 89, HR-10000 Zagreb, Croatia; a 89, HR-10000 Zagreb, Croatia; "> 3: Institute "Ruder Bokovi", P. O. BOX 180, HR-10002 Zagreb, Croatia; University of Zagreb, Faculty of Science, Department of Molecular Biology, Rooseveltov trg 6, HR-10000 Zagreb, Croatia;kovi", P. O. BOX 180, HR-10002 Zagreb, Croatia; University of Zagreb, Faculty of Science, Department of Molecular Biology, Rooseveltov trg 6, HR-10000 Zagreb, Croatia;">

Publication date: 2001-12-01

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• In this Subject: Botany
• By this author: Mihaljevi S. ;  Peri M. ;  Jelaska S.

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