Plant regeneration via organogenesis from six families of loblolly pine

Authors: Tang W.¹; Ouyang F.²

Source: Plant Cell, Tissue and Organ Culture, Volume 58, Number 3, 1999 , pp. 223-226(4)

Publisher: Springer

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Abstract:

Organogenesis was induced in callus derived from mature zygotic embryos of six families of loblolly pine (Pinus taeda L.) within 24 weeks of culture. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg l^-1 indole-3-butyric acid (IBA) and 2 mg l^-1 6-benzyladenine (BA). The most suitable medium for root formation proved to be TE medium supplemented with 0.1 mg l^-1 IBA, 1 mg l^-1 BA, and 0.5 mg l^-1 gibberellic acid (GA_3). One hundred and sixty-nine regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1:1:1) soil mixture, and 98 plantlets survived in the field.

Keywords: organogenesis; Pinus taeda L.; plant regeneration

Language: English

Document Type: Regular paper

Affiliations: 1: Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, China; Present address: Forest Biotechnology Group, Department of Forestry, North Carolina State University, Raleigh, NC 27695, U 2: Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, China;

Publication date: 1999-01-01