@article {Munoz:July 1998:0167-4412:807,
	author = "Munoz F.J.",
	author = "Labrador E.",
	author = "Dopico B.",
	title = "Brassinolides promote the expression of a new Cicer arietinum beta-tubulin gene involved in the epicotyl elongation",
	journal = "Plant Molecular Biology",
	volume = "37",
	year = "July 1998",
	abstract = "<P>A cDNA clone, CanTUB, encoding a putative <IMG SRC="/iso-ents/isogrk1/bgr-s.gif" ALT="bgr">-tubulin protein was isolated from a cDNA library constructed from 5-day old chickpea (Cicer arietinum) epicotyls. Analysis of its deduced amino acid sequence showed all the typical structural motifs of plant <IMG SRC="/iso-ents/isogrk1/bgr-s.gif" ALT="bgr">-tubulins. Putative sequences for autoregulation and tubulin mRNA stability, GTP and Ca^2+/MAPs (microtubule-associated proteins) binding sites were present. Southern blot analysis of chickpea genomic DNA revealed that there are multiple <IMG SRC="/iso-ents/isogrk1/bgr-s.gif" ALT="bgr">-tubulin genes. The level of expression of <IMG SRC="/iso-ents/isogrk1/bgr-s.gif" ALT="bgr">-tubulin genes was correlated with the rate of growth in either seedlings and adult plants. The transcript levels of <IMG SRC="/iso-ents/isogrk1/bgr-s.gif" ALT="bgr">-tubulin genes were higher in actively elongating tissues such as etiolated epicotyls, roots and stem tissues of adult plants. Brassinolide-induced growth in chickpea epicotyls was accompanied by promotion of the expression of the gene coding for <IMG SRC="/iso-ents/isogrk1/bgr-s.gif" ALT="bgr">-tubulin.</P>",
	pages = "807-817(11)",
	url = "http://www.ingentaconnect.com/content/klu/plan/1998/00000037/00000005/00163157"
}