Brassinolides promote the expression of a new Cicer arietinum -tubulin gene involved in the epicotyl elongation

Authors: Muñoz F.J.; Labrador E.; Dopico B.

Source: Plant Molecular Biology, Volume 37, Number 5, July 1998 , pp. 807-817(11)

Publisher: Springer

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Abstract:

A cDNA clone, CanTUB, encoding a putative bgr -tubulin protein was isolated from a cDNA library constructed from 5-day old chickpea (Cicer arietinum) epicotyls. Analysis of its deduced amino acid sequence showed all the typical structural motifs of plant bgr -tubulins. Putative sequences for autoregulation and tubulin mRNA stability, GTP and Ca^2+/MAPs (microtubule-associated proteins) binding sites were present. Southern blot analysis of chickpea genomic DNA revealed that there are multiple bgr -tubulin genes. The level of expression of bgr -tubulin genes was correlated with the rate of growth in either seedlings and adult plants. The transcript levels of bgr -tubulin genes were higher in actively elongating tissues such as etiolated epicotyls, roots and stem tissues of adult plants. Brassinolide-induced growth in chickpea epicotyls was accompanied by promotion of the expression of the gene coding for bgr -tubulin.

Keywords: brassinolides; bgr -tubulin; Cicer arietinum; epicotyls; growth

Language: English

Document Type: Regular paper

Affiliations: 1: Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Salamanca, 37008 Salamanca, Spain

Publication date: 1998-07-01