In Vitro Cytotoxic Effects of Dibromodulcitol in 9L Rat Brain Tumor Cells

Authors: Pulst, S-M.¹; Levin, V.A.¹; Deen, D.F.²

Source: Pharmaceutical Research, Volume 3, Number 5, 1 October 1986 , pp. 302-306(5)

Publisher: Springer

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Abstract:

Dibromodulcitol (DBD) is a halogenated hexitol active against animal and human brain tumors in vivo. In aqueous solution, DBD is transformed to products with different cytotoxicities. We studied the in vitro activation and inactivation of DBD with a bioassay in 9L rat brain tumor cells. We developed a mathematical model to calculate the rate constants of activation and inactivation. The kinetics of the activation and inactivation of DBD transformation products in cell culture medium were exponential, with rate constants of 0.139 and 0.0189 hr^-1, respectively. The maximum cell kill was caused by DBD that had been preincubated in medium for 13-16 hr. Thus cell kill is not caused by parent drug but by active transformation products.

Keywords: dibromodulcitol; bioassay; cytotoxicity

Document Type: Research article

Affiliations: 1: Brain Tumor Research Center of the Department of Neurological Surgery, School of Medicine, University of California, San Francisco, California 94143. 2: Brain Tumor Research Center of the Department of Neurological Surgery, School of Medicine, University of California, San Francisco, California 94143. Department of Radiation Oncology, School of Medicine, University of California, San Francisco, C

Publication date: 1986-10-01