Effect of peroxynitrite on production of superoxide anion radicals and calcium homeostasis in cells of astroglial origin

Authors: Kulagova, T.¹; Semenkova, G.²; Kvacheva, Z.; Ripich, T.³; Koren', S.

Source: Neurophysiology, Volume 38, Numbers 5-6, September 2006 , pp. 338-343(6)

Publisher: Springer

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Abstract:

We studied the effect of a donor of peroxynitrite, SIN-1, on the morphological characteristics of interweaved rat C6 glioma cells, on menadione-induced production of superoxide anion radicals, and on the concentration of Ca²⁺ in these cells. In concentrations of 1.25·10⁻⁴ to 2.5·10⁻⁷ M, SIN-1 demonstrated cytotoxic and antimitogenic effects. This donor of peroxynitrite caused abnormal modifications of the size of C6 cells and the structure of cellular organelles, intensified in a dose-dependent manner the release of Ca²⁺ from cellular stores into the cytoplasm, and suppressed menadione-induced production of superoxide anion radicals. Therefore, it should be believed that peroxynitrite exerts a modifying effect on the processes of mitotic division and induces apoptosis; it is also involved in the processes of intracellular signalling providing an increase in the concentration of cytosolic Ca²⁺ and a decrease in the redox activity of cells.

Keywords: interweaved C6 glioma cells of rat; peroxynitrite; menadione-dependent chemiluminescence; cytoplasmic concentration of Ca2+

Document Type: Research article

DOI: 10.1007/s11062-006-0068-0

Affiliations: 1: Email: tatyana_kulagova@tut.by 2: Email: galina_semenkova@yahoo.com 3: Email: ripich@mail.ru